|Publication Year: 2019|
Highlight ID: 1335
|Patterns of Gene Expression Provide Insight into Wood Decay Mechanisms|
Comparative examination of gene regulation reveal significant differences among brown rot fungi when colonizing different wood species. ...
|Publication Year: 2017|
Highlight ID: 1296
|Enzymatic conversion of xylan into valuable products.|
Xylan represents a vast store of potential value but rather than seeing beneficial use, much of this reservoir is lost in catastrophic wildfires that result in significant property loss and tragic los ...
Highlight ID: 1295
|Genetic basis of lignocellulose degradation|
Exploiting the recent availability of fungal genomes, Forest Service researchers have developed computational approaches for the identification of key wood decay enzymes. ...
|Publication Year: 2016|
Highlight ID: 663
|Characterization of Microbial Biocatalysts in Lignocellulosic Utilization|
Bioconversion of lignocellulosic biomass would benefit from development of second generation bacterial biocatalysts. The bacterium Paenibacillus sp. strain JDR2, originally isolated from decaying swee ...
Highlight ID: 662
|Development of Enzymes for use in Lignocellulose Processing|
The ultimate best use of lignocellulose allows for the selective extraction of defined value streams. To facilitate this, Forest Service research strives to develop new enzymes with unique capabilitie ...
Highlight ID: 665
|Fungi Use Multiple Strategies for Deconstruction of Woody Biomass|
A diverse array of enzymes catalyze the bioconversion of wood toward biofuels and other high-value products. ...
Highlight ID: 653
|Improving the Hydrolysis and Fibrillation of Wood Into Cellulose Nanomaterials|
Cellulose nanomaterials have been receiving an increasing amount of interest from both the scientific and industrial communities because of their interesting properties, including good strength, absor ...
|Publication Year: 2015|
Highlight ID: 618
|How Does Wood Decay Start?|
How do fungi rot wood? Using new tools, fluorescent dye attached to tiny beads, and laser confocal microscopy, Forest Service scientists were able to follow oxidation on a microscopic level. The infor ...
Highlight ID: 620
|Imaging Wood Chemicals in Three Dimensions|
For the first time, chemicals in wood were visualized in 3-dimensions. This advance in chemical analysis will help clarify scientists' understanding of wood architecture, strength properties, durabili ...
Highlight ID: 613
|Protein Structure and Biochemical Characterization of a Novel Functioning Xylanase|
Scientists identified and characterized a xylanase with unique function that may have applications in processing of woody biomass substrate. ...
|Publication Year: 2013|
Highlight ID: 445
|Common Button Mushroom Studied for Key Role in Recycling Carbon|
Recent genome studies of Agaricus reveal specialized adaptations to litter- and soil-rich environments which may be used to remediate contaminated soils. ...
Highlight ID: 437
|Enzyme Combo Results in Bioplastics Composite|
Developing bioplastics as a means of moving away from non-renewable, petroleum-based products has many economic and environmental benefits. Researchers at the Forest Products Laboratory recently devel ...
Highlight ID: 446
|Research Demystifies Wood Decaying Fungi|
Newly sequenced fungal genomes provide insight into the enzymatic conversion of wood into high value products. ...
Highlight ID: 452
|Researchers Determine the Structure of Bacterial Protein Involved in Biomass Conversion|
Researchers structurally characterized a bacterial protein involved in biomass degradation. The knowledge obtained from this novel protein suggest that it may function better than comparable enzymes i ...
|Publication Year: 2012|
Highlight ID: 9
|Hidden Lives of Wood Decay Fungi Uncovered by Genome Sequencing|
The decoded genomes of 12 species reveal a complex repertoire of proteins involved in the deconstruction of key polymers within wood cell walls ...
Highlight ID: 11
|Novel Yeast Makes Bioconversion Faster and Less Expensive|
Faster, cheaper biofuel production is possible for commercial use ...
|Publication Year: 2010|
Highlight ID: 178
|Fast Forward' Genetics for Renewable Fuels|
Researchers at the Forest Products Laboratory used mutagenesis, strain selection and genetic manipulation over a period of seven years to develop improved strains of yeasts that will produce renewable ...
Highlight ID: 176
|Water, Air, and Soil|
Forest fungi perform an essential role in recycling woody plant debris and have many potential applications in biotechnology. In 2009, a Forest Products Laboratory chemist worked for ten months as a S ...
Crooks, M.E. Casey
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Kersten, Philip J.
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St. John, Franz
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|Below are the 50 most recent products author or co-authored by researchers of this unit that have been posted, sorted by publication year and title. To access the complete repository of FPL products, click here.|
|Publication Year||Title||Date Posted|
|2020||CaXyn30B from the solventogenic bacterium Clostridium acetobutylicum is a glucuronic acid-dependent endoxylanase||09/18/20|
|2020||Characterization and functional analysis of two novel thermotolerant α-l-arabinofuranosidases belonging to glycoside hydrolase family 51 from Thielavia terrestris and family 62 from Eupenicillium parvum||01/08/21|
|2020||Conserved white-rot enzymatic mechanism for wood decay in the Basidiomycota genus Pycnoporus||09/04/20|
|2020||Kinetic characterization and structure analysis of an altered polyol dehydrogenase with d ‐lactate dehydrogenase activity||01/06/21|
|2020||Lignin lags, leads, or limits the decomposition of litter and soil organic carbon||10/02/20|
|2020||Mechanism of extractive degradation by Phlebiopsis gigantea||09/30/20|
|2019||Enrichment of lignin-derived carbon in mineral-associated soil organic matter||05/28/20|
|2019||Evolution of substrate-specific gene expression and RNA editing in brown rot wood-decaying fungi||10/01/19|
|2019||The foliar endophyte Phialocephala scopiformis DAOMC 229536 proteome when grown on wood used as the sole carbon source||10/01/19|
|2019||Xylanase pretreatment of wood fibers for producing cellulose nanofibrils: a comparison of different enzyme preparations||09/05/19|
|2018||A plasmid borne, functionally novel glycoside hydrolase family 30 subfamily 8 endoxylanase from solventogenic Clostridium||12/20/18|
|2018||Draft genome sequence of Burkholderia cepacia ATCC 17759, a polyhydroxybutyrate-co-valerate copolymer-producing bacterium||10/01/18|
|2018||Fungal lignin peroxidase does not produce the veratryl alcohol cation radical as a diffusible ligninolytic oxidant||07/26/18|
|2018||Multi-omic analyses of extensively decayed Pinus contorta reveal expression of a diverse array of lignocellulose-degrading enzymes||10/02/18|
|2018||Substrate-specific differential gene expression and RNA editing in the brown rot fungus Fomitopsis pinicola||09/28/18|
|2017||An optical method for carbon dioxide isotopes and mole fractions in small gas samples: tracing microbial respiration from soil, litter, and lignin.||10/05/17|
|2017||Draft genome sequence of a monokaryotic model brown-rot fungus Postia (Rhodonia) placenta SB12||09/06/17|
|2017||Exploring the hypothesis that limiting diffusion of fungal oxidants underlies decay resistance in acetylated wood||10/06/17|
|2017||GH115 α-glucuronidase and GH11 xylanase from Paenibacillus sp. JDR-2: potential roles in processing glucuronoxylans||08/31/18|
|2017||GH30 glucuronoxylan-specific xylanase from Streptomyces turgidiscabies C56||12/20/18|
|2017||Xylanase 30 A from Clostridium thermocellum functions as a glucuronoxylan xylanohydrolase||10/06/17|
|2016||A 1,3-1,4-β-glucan utilization regulon in Paenibacillus sp. strain JDR-2||09/30/16|
|2016||Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi||09/07/16|
|2016||Construction of a genetic linkage map and analysis of quantitative trait loci associated with the agronomically important traits of Pleurotus eryngii||07/12/16|
|2016||Draft genome sequence of the white-rot fungus Obba rivulosa 3A-2||09/29/16|
|2016||Endoglucanase post-milling treatment for producing cellulose nanofibers from bleached eucalyptus fibers by a supermasscolloider||07/15/16|
|2016||Gene expression patterns of wood decay fungi Postia placenta and Phanerochaete chrysosporium are influenced by wood substrate composition during degradation||09/22/16|
|2016||Genetic bases of fungal white rot wood decay predicted by phylogenomic analysis of correlated gene-phenotype evolution||10/06/17|
|2016||Genomic and transcriptomic analysis of carbohydrate utilization by Paenibacillus sp. JDR-2: systems for bioprocessing plant polysaccharides||09/30/16|
|2016||Glycosyl Hydrolase Xylanases, Compositions and Methods of Use for Efficient Hydrolysis and Processing of Xylan *||05/23/16|
|2016||Heterologous production and characterization of two glyoxal oxidases from Pycnoporus cinnabarinus||09/22/16|
|2016||Iron addition to soil specifically stabilized lignin||05/24/16|
|2016||Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta||07/05/17|
|2016||Prospects for bioprocess development based on recent genome advances in lignocellulose degrading basidiomycetes||09/29/16|
|2016||Transcriptome and secretome analyses of the wood decay fungus Wolfiporia cocos support alternative mechanisms of lignocellulose conversion||09/22/16|
|2015||Basidiomycete DyPs: Genomic diversity, structural-functional aspects, reaction mechanism and environmental significance||12/07/15|
|2015||Chemistry of wood in 3D: new infrared imaging||12/15/15|
|2015||Contrasting Patterns of Diterpene Acid Induction by Red Pine and White Spruce to Simulated Bark Beetle Attack, and Interspecific Differences in Sensitivity Among Fungal Associates||07/07/15|
|2015||Lignin decomposition is sustained under fluctuating redox conditions in humid tropical forest soils||09/25/15|
|2015||Physical and Mechanical Properties of Cellulose Nanofibril Films from Bleached Eucalyptus Pulp by Endoglucanase Treatment and Microfluidization||09/25/15|
|2015||Production of cellulose nanofibrils from bleached eucalyptus fibers by hyperthermostable endoglucanase treatment and subsequent microfluidization||02/11/15|
|2015||Prospects and challenges for fungal metatranscriptomics of complex communities||02/11/15|
|2015||Regulation of Gene Expression during the Onset of Ligninolytic Oxidation by Phanerochaete chrysosporium on Spruce Wood||12/15/15|
|2014||2D-NMR Demonstration of Lignin Removal from Wood and Non-wood plant Feedstocks by Fungal Versatile Peroxidase||10/01/14|
|2014||3D FT-IR imaging spectroscopy of phase-separation in a poly(3-hydroxybutyrate)/poly(L-lactic acid) blend||07/20/15|
|2014||A Highly Diastereoselective Oxidant Contributes to Ligninolysis by the White Rot Basidiomycete Ceriporiopsis subvermispora||10/01/14|
|2014||A novel member of glycoside hydrolase family 30 subfamily 8 with altered substrate specificity||07/16/15|
|2014||Analysis of the Phlebiopsis gigantea Genome, Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood||12/22/14|
|2014||Chapter 3: Wood Decay||09/16/14|
|2014||Copper radical oxidases and related extracellular oxidoreductases of wood-decay Agaricomycetes||09/17/14|
|2014||Disruption of the Cytochrome C Gene in Xylose-Fermenting Yeast *||09/22/14|
|2014||Engineering the Xylan Utilization System in Bacillus subtilis for Production of Acidic Xylooligosaccharides||09/22/14|
|2014||Ethanol production from non-detoxified whole slurry of sulfite-pretreated empty fruit bunches at a low cellulase loading||07/18/14|
|2014||Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungi||09/17/14|
|2014||Fungal Degradation and Bioremediation System for ACQ-Treated Wood *||09/19/14|
|2014||Fungal Degradation and Bioremediation System for CCA-Treated Wood *||09/25/14|
|2014||Fungal Degradation and Bioremediation System for CCA-Treated Wood *||09/29/14|
|2014||Fungal Degradation and Bioremediation System for Creosote-Treated Wood *||09/19/14|
|2014||Fungal Degradation and Bioremediation System for Creosote-Treated Wood *||09/25/14|
|2014||Fungal Degradation and Bioremediation System for Pentachlorophenol-Treated Wood *||09/19/14|
|2014||Fungal Degradation and Bioremediation System for Pentachlorophenol-Treated Wood *||09/25/14|
|2014||Fungal Inocolum Preparation *||09/22/14|
|2014||Fungal Inoculum Preparation *||09/22/14|
|2014||Influence of Populus Genotype on Gene Expression by the Wood Decay Fungus Phanerochaete chrysosporium||09/17/14|
|2014||Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability||09/16/14|
|2014||Method for Producing Pulp *||09/29/14|
|2014||Method of Pulping Wood Chips with a Fungi Using Sulfite Salt-Treated Wood Chip *||09/22/14|
|2014||Method of Removing Color From Kraft Wood Pulps *||09/22/14|
|2014||Method of Removing Color From Wood Pulp Using Xylanase From Streptomyces Roseiscleroticus NRRL-B-11019 *||09/22/14|
|2014||Porphyrins and Uses Thereof *||09/23/14|
|2014||Sham-Sensitive Terminal Oxidase Gene from Xylose-Fermenting Yeast *||09/22/14|
|2014||Solid Phase Bioremediation Methods Using Lignin-Degrading Fungi *||09/22/14|
|2014||Temporal Alterations in the Secretome of the Selective Ligninolytic Fungus Ceriporipsis subvermispora during growth on Aspen Wood Reveal this Organism's Strategy for Degrading Lighnocellulose||09/17/14|
|2014||Transcriptomic Analysis of Xylan Utilization Systems in Paenibacillus sp||07/20/15|
|2014||What Basidiomycete Genomes Teach us about Wood Biodegradation Mechanisms||10/01/14|
|2014||Xylanase From Streptomyces Roseiscleroticus NRRL-11019 for Removing Color From Kraft Wood Pulps *||09/23/14|
|2014||Xylose-Fermenting Recombinant Yeast Strains *||09/25/14|
|2014||Xylose-Fermenting Recombinant Yeast Strains *||09/25/14|
|2014||Xylose-Fermenting Yeast Mutants *||09/23/14|
|2014||Yeast Transformation System *||05/29/14|
|2013||3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography||10/20/14|
|2013||A comparative genomic analysis of the oxidative enzymes potentially involved in lignin degradation by Agaricus bisporus||09/05/13|
|2013||Chapter 13: Recent Advances on the Genomics of Litter- and Soil-Inhabiting Agaricomycetes||09/30/13|
|2013||Chapter 5: Organopollutant Degradation by Wood Decay Basidiomycetes||11/18/14|
|2013||Chapter 6: Prehydrolysis Pulping with Fermentation Coproducts||09/30/13|
|2013||Chemically imaging the effects of the addition of nanofibrillated cellulose on the distribution of poly(acrylic acid) in poly(vinyl alcohol)||04/02/13|
|2013||Co-Fermentation of Glucose, Xylose and/or Cellobiose by Yeast *||10/21/13|
|2013||Differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from Burkholderia cepacia and Pseudomonas pseudoflava||08/02/13|
|2013||Evidence from Serpula lacrymans that 2,5-Dimethoxyhydroquinone Is a Lignocellulolytic Agent of Divergent Brown Rot Basidiomycetes||09/05/13|
|2013||Formation of a tyrosine adduct involved in lignin degradation by Trametopsis cervina lignin peroxidase: a novel peroxidase activation mechanism||09/09/13|
|2013||Genomewide analysis of polysaccharides degrading enzymes in 11 white- and brown-rot Polyporales provides insight into mechanisms of wood decay||09/09/14|
|2013||Integration of Chemical and Biological Catalysis: Production of Furylglycolic Acid from Glucose via Cortalcerone||02/24/14|
|2013||Method of Making Medium Density Fiberboard *||08/15/13|
|2013||Nondestructive chemical imaging of wood at the micro-scale: advanced technology to complement macro-scale evaluations||10/24/13|
|2013||Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX pretreated corn stover||09/13/13|
|2013||Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism||05/14/13|
|2013||Sugar Transport Sequences, Yeast Strains Having Improved Sugar Uptake, and Methods of Use *||08/15/13|
|2013||Xylanase supplementation on enzymatic saccharification of dilute acid pretreated poplars at different severities||10/22/13|
|2012||Bioconversion of giant reed (Arundo donax L.) hemicellulose hydrolysate to ethanol by Scheffersomyces stipitis CBS6054||09/27/12|
|Below are 12 research projects associated with this research unit.|
|Project Number||Title||Project Dates|
|FPL-4712-1-T||Bioconversion||07-31-2002 - 07-30-2007|
|FPL-4712-4A||Bioconversion of thinnings||07-23-2007 - 07-22-2012|
|FPL-4712-1B||Bioconversion of wood sugars to fuels and other chemicals||10-01-2012 - 09-30-2019|
|FPL-4712-1A||Bioconversion of wood sugars to fuels and other chemicals||07-23-2007 - 07-22-2012|
|FPL-4712-3A||Enzymatic and microbial processing of wood and wood fiber to fuels and other chemicals||07-23-2007 - 07-22-2012|
|FPL-4712-3B||Enzymatic and microbial processing of wood and wood fiber to fuels, nanocellulose and other chemicals||10-01-2012 - 09-30-2019|
|FPL-4712-3-T||Enzymatic processing of wood fiber||07-31-2002 - 07-30-2007|
|FPL-4712-4-T||Optimize lignocelluose-degrading fungi for bioprocessing and bioconversion of wood||07-31-2002 - 07-30-2007|
|FPL-4712-4B||Optimizing lignocellulose-degrading fungi for bioprocessing and bioconversion of wood requires understanding the functional genomics of fung||10-01-2012 - 09-30-2019|
|FPL-4712-2A||Oxidative systems of wood decay fungi||07-23-2007 - 07-22-2012|
|FPL-4712-2B||Oxidative systems of wood decay fungi||10-01-2012 - 09-30-2019|
|FPL-4712-2-T||Oxidative systems of wood decay fungi||07-31-2002 - 07-30-2007|
|Making Ethanol from Biomass to Meet Energy Demands|
Forest Products Laboratory (FPL) researchers have had proven and continuing success working with commercial partners to improve methods of converting biomass to alternative fuels such as ethanol. One important goal of these collaborations is to promote biomass as a way to meet increasing worldwide energy demands.
Xethanol Corporation, an ethanol producer with facilities in Iowa, acquired licensing rights to a patented process developed by FPL and the University of Wisconsin and signed a cooperative research and development agreement to solidify the partnership. The patented process aids in the conversion of xylose, a sugar found in waste biomass and corn fiber, to ethanol and xylitol, a sweetener used in food processing.
The conversion takes place through a fermentation process in which specialized yeast strains break down the xylose. The process reduces the time and labor involved in the critical task of isolating and screening yeast strains for effective fermentation agents and can be used for conversion of both agricultural waste and woody biomass.
Collaborative research in this area is continuing with a new partnership recently formed between FPL and EdeniQ, a company that focuses on turning abundant, non-food biomass into fuels. Researchers are developing yeasts used to ferment xylose and glucose from biomass for commercial ethanol production.
Below are the 2 most recent Lab Notes blog postings pertaining to this research unit.
|Genetics Provide Valuable Insight into Mysterious Decay Fungi|
Valuable insights to developing effective biological control agents for protecting conifer trees from root rot have been discovered...
|Pulp NonFiction: Fungal Analysis Reveals Clues for Targeted Biomass Deconstruction|
Without fungi and microbes to break down dead trees and leaf litter in nature, the forest floor might look like a scene from TV's "Hoarders." Dan Cullen, research microbiologist at the FPL, is part of an international team of scientists studying the ...