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Title: Fungal–copper interactions in wood examined with large field of view synchrotron-based X-ray fluorescence microscopy

Source: Wood Material Science & Engineering. 16(5): 1-11.

Author(s)Zelinka, Samuel L.; Jakes, Joseph E.; Tang, Juliet ; Ohno, Katie , M.; Bishell, Amy ; Finney, Lydia ; Maxey, Evan R.; Vogt, Stefan ; Kirker, Grant T.

Publication Year: 2018  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4707-2B   FPL-4716-3A   FPL-4723-1A

Abstract: The goal of this study was to demonstrate how synchrotron-based X-ray fluorescence microscopy (XFM) can be used to better understand the mechanisms of copper tolerance in wood decay fungi. Copper is a major component in commercial wood preservatives as it is toxic to many wood decay fungi. However, certain fungi are copper tolerant and can attack preservative-treated wood, resulting in structural damage to treated wood members. Here we used large-field XFM to visualize six different elements (K, Ca, Mn, Fe, Cu, and Zn) in the mycelia and wood inoculated with four different species of brown rot wood decay fungi. Wood blocks were partially dipped into a solution of copper sulfate, exposed to fungi in malt extract agar petri dish assays for nine weeks, and then imaged and compared to blocks that were partially dipped in water. The blocks were imaged immediately adjacent to an end-matched control that was placed in malt extract agar petri dish assays for 9 weeks, but not exposed to the fungi so that the differences in the elemental distributions could be directly compared. The colonized wood and mycelia were rich in K, Ca, Mn, and Fe; however, the elements and the spatial distribution in the mycelia and wood differed across fungal species. The most interesting results were the maps showing the copper distribution. While three of the four fungi grew on the copper-rich region of the wood, only one species, Fibroporia radiculosa, dramatically reduced the copper concentration in the region of fungal growth.

Keywords: Wood decay; brown-rot fungi; Fibroporia radiculosa; ion translocation

Publication Review Process: Formally Refereed

File size: 3,072 kb(s)

Date posted: 07/26/2018

This publication is also viewable on Treesearch:  view
RITS Product ID: 90733
Current FPL Scientists associated with this product (listed alphabetically)
Jakes, Joseph
Research Materials Engineer
Kirker, Grant
Research Forest Products Technologist
Ohno, Katie
Research Biologist
Tang, Juliet
Research Forest Products Technologist
Zelinka, Samuel L.
Materials Research Engineer

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