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Title: Analysis of hindgut bacterial phyla frequency and diversity in subterranean termites exposed to chitosan-treated wood

Source: The International Research Group on Wood Protection, Section 1, Biology: Paper prepared for the IRG48 Scientific Conference on Wood Protection. Ghent, Belgium: 2-8.

Author(s)Raji, Olanrewaju; Tang, Juliet D.; Telmadarrehei, Telmah; Jeremic, Dragica

Publication Year: 2017  View PDF »

Category: Conference Proceedings
Associated Research Project(s):   FPL-4723-2A

Abstract: The termite hindgut contains a microbial community that symbiotically aids in digestion of lignocellulosic materials. For better understanding of the dynamics of the bacteria-termite relationship, a species survey of bacterial hindgut microbes in subterranean termites (Reticulitermes flavipes: Kollar) collected from Louisville, Mississippi was performed after exposure to chitosan-treated and control (water-treated) wood samples. Total genomic DNA was isolated from termite hindguts, amplified and 16S ribosomal RNA (rRNA) gene fragments were analyzed using next-generation sequencing techniques. Twenty-six bacteria phyla were identified in both treatment groups, with five bacteria phyla showing significantly differences in abundance between the chitosan-treated and control groups. These results suggest that there was a treatment driven effect on the hindgut bacteria diversity.

Keywords: Metagenomics; Reticulitermes flavipes (Isoptera: Rhinotermitidae); hindgut; bacteria; chitosan; wood

Publication Review Process: Informally Refereed (Peer-Reviewed)

File size: 647 kb(s)

Date posted: 08/23/2017

This publication is also viewable on Treesearch:  view
RITS Product ID: 85520
Current FPL Scientist associated with this product
Tang, Juliet
Research Forest Products Technologist
  

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