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Title: Synchrotron-based X-ray fluorescence microscopy enables multiscale spatial visualization of ions involved in fungal lignocellulose deconstruction

Source: Scientific Reports, Vol. 7:1-15.

Author(s)Kirker, Grant T.; Zelinka, Samuel; Gleber, Sophie-Charlotte; Vine, David; Finney, Lydia; Chen, Si; Hong, Young Pyo; Uyarte, Omar; Vogt, Stefan; Jellison, Jody; Goodell, Barry; Jakes, Joseph E.

Publication Year: 2017  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4707-2B  FPL-4716-3A  FPL-4723-1A

Abstract: The role of ions in the fungal decay process of lignocellulose biomaterials, and more broadly fungal metabolism, has implications for diverse research disciplines ranging from plant pathology and forest ecology, to carbon sequestration. Despite the importance of ions in fungal decay mechanisms, the spatial distribution and quantification of ions in lignocellulosic cell walls and fungal hyphae during decay is not known. Here we employ synchrotron-based X-ray fluorescence microscopy (XFM) to map and quantify physiologically relevant ions, such as K, Ca, Mn, Fe, and Zn, in wood being decayed by the model brown rot fungus Serpula lacrymans. Two-dimensional XFM maps were obtained to study the ion spatial distributions from mm to submicron length scales in wood, fungal hyphae with the dried extracellular matrix (ECM) from the fungus, and Ca oxalate crystals. Three-dimensional ion volume reconstructions were also acquired of wood cell walls and hyphae with ECM. Results show that the fungus actively transports some ions, such as Fe, into the wood and controls the distribution of ions at both the bulk wood and cell wall length scales. These measurements provide new insights into the movement of ions during decay and illustrate how synchrotron-based XFM is uniquely suited study these ions.

Keywords: X-ray fluorescence microscopy, Brown rot fungi, Wood decay, Tomography

Publication Review Process: Formally Refereed

File size: 3,072 kb(s)

Date posted: 03/01/2017

This publication is also viewable on Treesearch:  view
RITS Product ID: 84183
Current FPL Scientists associated with this product (listed alphabetically)
Jakes, Joseph
Research Materials Engineer
Kirker, Grant
Research Forest Products Technologist
Zelinka, Samuel L.
Materials Research Engineer

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