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Title: Transcriptome and secretome analyses of the wood decay fungus Wolfiporia cocos support alternative mechanisms of lignocellulose conversion

Source: Applied and Environmental Microbiology, 82(13): 3979-3987

Author(s)Gaskell, Jill; Blanchette, Robert A.; Stewart, Philip E.; BonDurant, Sandra Splinter; Adams, Marie; Sabat, Grzegorz; Kersten, Philip; Cullen, Daniel

Publication Year: 2016  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4712-4B

Abstract: Certain wood decay basidiomycetes, collectively referred to as brown rot fungi, rapidly depolymerize cellulose while leaving behind the bulk of cell wall lignin as a modified residue. The mechanism(s) employed is unclear, but considerable evidence implicates the involvement of diffusible oxidants generated via Fenton-like chemistry. Toward a better understanding of this process, we have examined the transcriptome and secretome of Wolfiporia cocos when cultivated on media containing glucose, purified crystalline cellulose, aspen (Populus grandidentata), or lodgepole pine (Pinus contorta) as the sole carbon source. Compared to the results obtained with glucose, 30, 183, and 207 genes exhibited 4-fold increases in transcript levels in cellulose, aspen, and lodgepole pine, respectively. Mass spectrometry identified peptides corresponding to 64 glycoside hydrolase (GH) proteins, and of these, 17 corresponded to transcripts upregulated on one or both woody substrates. Most of these genes were broadly categorized as hemicellulases or chitinases. Consistent with an important role for hydroxyl radical in cellulose depolymerization, high transcript levels and upregulation were observed for genes involved in iron homeostasis, iron reduction, and extracellular peroxide generation. These patterns of regulation differ markedly from those of the closely related brown rot fungus Postia placenta and expand the number of enzymes potentially involved in the oxidative depolymerization of cellulose.

Keywords: Wolfiporia, brown rot, cellulose, transcriptome, secretome

Publication Review Process: Formally Refereed

File size: 381 kb(s)

Date posted: 09/22/2016

This publication is also viewable on Treesearch:  view
RITS Product ID: 81733
Current FPL Scientists associated with this product (listed alphabetically)
Cullen, Daniel
Research Microbiologist
Kersten, Philip J.
Research Microbiologist
 

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