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Title: Heterologous production and characterization of two glyoxal oxidases from Pycnoporus cinnabarinus

Source: Applied and Environmental Microbiology, 82(16)

Author(s)Daou, Marianne; Piumi, François; Cullen, Daniel; Record, Eric; Faulds, Craig B.

Publication Year: 2016  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4712-4B

Abstract: The genome of the white rot fungus Pycnoporus cinnabarinus includes a large number of genes encoding enzymes implicated in lignin degradation. Among these, three genes are predicted to encode glyoxal oxidase, an enzyme previously isolated from Phanerochaete chrysosporium. The glyoxal oxidase of P. chrysosporium is physiologically coupled to lignin-oxidizing peroxidases via generation of extracellular H2O2 and utilizes an array of aldehydes and -hydroxycarbonyls as the substrates. Two of the predicted glyoxal oxidases of P. cinnabarinus, GLOX1 (PciGLOX1) and GLOX2 (PciGLOX2), were heterologously produced in Aspergillus niger strain D15#26 (pyrG negative) and purified using immobilized metal ion affinity chromatography, yielding 59 and 5 mg of protein for PciGLOX1 and PciGLOX2, respectively. Both proteins were approximately 60 kDa in size and N-glycosylated. The optimum temperature for the activity of these enzymes was 50°C, and the optimum pH was 6. The enzymes retained most of their activity after incubation at 50°C for 4 h. The highest relative activity and the highest catalytic efficiency of both enzymes occurred with glyoxylic acid as the substrate. The two P. cinnabarinus enzymes generally exhibited similar substrate preferences, but PciGLOX2 showed a broader substrate specificity and was significantly more active on 3-phenylpropionaldehyde.

Keywords: Pycnoporus, glyoxal oxidases, lignin, white rot

Publication Review Process: Formally Refereed

File size: 1,024 kb(s)

Date posted: 09/22/2016

This publication is also viewable on Treesearch:  view
RITS Product ID: 81732
Current FPL Scientist associated with this product
Cullen, Daniel
Research Microbiologist
  

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