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Title: Pilot-scale demonstration of SPORL for bioconversion of lodgepole pine to bioethanol and lignosulfonate

Source: Holzforschung

Author(s)Zhou, Haifeng; Zhu, Junyong; Gleisner, Roland; Qiu, Xueqing; Horn, Eric; Negron, Jose

Publication Year: 2016  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4709-1B

Abstract: The process sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) has been the focus of this study. Pilot-scale (50 kg) pretreatment of wood chips of lodgepole pine (Pinus contorta Douglas ex Loudon) killed by mountain pine beetle (Dendroctonus ponderosae Hopkins) were conducted at 165°C with a dilute sulfite solution of pH 2 for bioconversion to ethanol and lignosulfonate (LS). The pretreatment duration was optimized in laboratory bench scale experiments with a certain severity based on a combined hydrolysis factor (CHF). The sodium bisulfite loading was 8% and the liquor to wood ratio 3. The pretreated solids were disk milled together with the spent liquid and the resultant slurry with a 25% solids content was directly (without detoxification) submitted to a simultaneous enzymatic saccharification and fermentation (SSF) with Saccharomyces cerevisiae YRH400 at cellulase loading of 35 ml kg-1 of untreated wood. At solids loading of 20%, the alcohol yield was 288 l t-1 wood (with a final concentration of 52.2 g l-1), which corresponds to a 72.0% theoretical yield based on total glucan, mannan, and xylan. The LS from SPORL was highly sulfonated and its molecular weight was lower than that of a purified commercial softwood LS, and therefore it has a high potential as a directly marketable co-product.

Keywords: biofuel; enzymatic saccharification/hydrolysis; forest biorefinery; high solids fermentation; lignosulfonate; process scale-up

Publication Review Process: Formally Refereed

File size: 1,024 kb(s)

Date posted: 07/28/2016

This publication is also viewable on Treesearch:  view
RITS Product ID: 79756
Current FPL Scientist associated with this product
Zhu, JunYong
Research General Engineer

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