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Forest Products Laboratory
One Gifford Pinchot Drive
Madison, WI 53726-2398
Phone: (608) 231-9200
Fax: (608) 231-9592


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Title: Endoglucanase post-milling treatment for producing cellulose nanofibers from bleached eucalyptus fibers by a supermasscolloider

Source: Cellulose

Author(s)Wang, Wangxia; Mozuch, Michael D.; Sabo, Ronald C.; Kersten, Philip; Zhu, Junyong; Jin, Yongcan

Publication Year: 2016  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4706-2B   FPL-4709-2B   FPL-4712-3B

Abstract: Three recombinant GH5 endoglucanases chosen for their contrasting hydrolytic activities, and a commercial endoglucanase were used to treat cellulose nanofibers (CNFs) after they were milled from bleached eucalyptus pulp with a supermasscolloider. This enzyme ‘‘post-treatment’’ resulted in different properties for the CNFs depending on enzyme treatment. The degree of polymerization, water retention value and enzymatic digestibility were used to estimate the extent of fibrillation. Morphologies of CNFs were observed by SEM and TEM. Electron microscopy images indicated endoglucanase posttreatment could efficiently improve the nanosize uniformity of CNFs from supermasscolloider fibrillation. All GH5 endoglucanase post-treated CNFs had a diameter between 3 and 19 nm with 70–89 %between 5 and 9 nm. The recombinant GH5 with no reported activity on crystalline cellulose was the most aggressive of the GH5s studied in reducing the DP of CNFs.

Keywords: Endoglucanase; Post-treatment; Cellulose nanofibers; Fibril morphology; Disk grinding/fibrillation; Endoglucanase; Post-treatment; Cellulose nanofibers; Fibril morphology; Disk grinding/fibrillation

Publication Review Process: Formally Refereed

File size: 6,144 kb(s)

Date posted: 07/15/2016

This publication is also viewable on Treesearch:  view
RITS Product ID: 79636
Current FPL Scientists associated with this product (listed alphabetically)
Kersten, Philip J.
Research Microbiologist
Sabo, Ronald C.
Research Materials Engineer
Zhu, JunYong
Research General Engineer

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