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Title: Transcriptomic Analysis of Xylan Utilization Systems in Paenibacillus sp

Source: Applied and Environmental Microbiology

Author(s)Sawhney, Neha; Crooks, Casey; St. John, Franz; Preston, James F.; Kelly, R. M.

Publication Year: 2014  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4712-1B

Abstract: Xylans, including methylglucuronoxylans (MeGXn) and methylglucuronoarabinoxylans (MeGAXn), are the predominant polysaccharides in hemicellulose fractions of dicots and monocots available for conversion to biofuels and chemicals. Paenibacillus sp. strain JDR-2 (Pjdr2) efficiently depolymerizes MeGXn and MeGAXn and assimilates the generated oligosaccharides, resulting in efficient saccharification and subsequent metabolism of these polysaccharides. A xylan utilization regulon encoding a cell associated GH10 (glycoside hydrolase family 10) endoxylanase, transcriptional regulators, ABC (ATP binding cassette) transporters, an intracellular GH67 α-glucuronidase, and other glycoside hydrolases contributes to complete metabolism. This GH10/GH67 system has been proposed to account for preferential utilization of xylans compared to free oligo- and monosaccharides. To identify additional genes contributing to MeGXn and MeGAXn utilization, the transcriptome of Pjdr2 has been sequenced following growth on each of these substrates as well as xylose and arabinose. Increased expression of genes with different substrates identified pathways common or unique to the utilization of MeGXn or MeGAXn. Coordinate upregulation of genes comprising the GH10/GH67 xylan utilization regulon is accompanied with upregulation of genes encoding a GH11 endoxylanase and a GH115 α-glucuronidase, providing evidence for a novel complementary pathway for processing xylans. Elevated expression of genes encoding a GH43 arabinoxylan arabinofuranohydrolase and an arabinose ABC transporter on MeGAXn but not on MeGXn supports a process in which arabinose may be removed extracellularly followed by its rapid assimilation. Further development of Pjdr2 for direct conversion of xylans to targeted products or introduction of these systems into fermentative strains of related bacteria may lead to biocatalysts for consolidated bioprocessing of hemicelluloses released from lignocellulose.

Keywords: Xylan; Transcriptome; Paenibacillus; lignocellulose

Publication Review Process: Formally Refereed

File size: 867 kb(s)

Date posted: 07/20/2015

This publication is also viewable on Treesearch:  view
RITS Product ID: 73347
Current FPL Scientists associated with this product (listed alphabetically)
Crooks, M.E. Casey
Research Microbiologist
St. Johns, Franz
Research Microbiologist

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