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Forest Products Laboratory
One Gifford Pinchot Drive
Madison, WI 53726-2398
Phone: (608) 231-9200
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Title: 2D-NMR Demonstration of Lignin Removal from Wood and Non-wood plant Feedstocks by Fungal Versatile Peroxidase

Source: In: EWLP 2014, 13th European Workshop on Lignocellulosics and Pulp, Proceedings 24-27 June 2014, Seville, Spain. pp. 347-350.

Author(s)Fernandez-Fueyo, Elena; Recoret, Jorge; Berbis, M. Alvaro; Ruiz-Duenas, Francisco J.; Gutierrez, Ana; del Rio, Jose C.; Jimenez-Barbero, Jesus; Hammel, Kenneth E.; Martinez, Angel T.

Publication Year: 2014  View PDF »

Category: Conference Proceedings
Associated Research Project(s):   FPL-4712-2B

Abstract: The ability of versatile peroxidase to modify/degrade lignin (in conjunction with veratryl alcohol as a mediator) in both wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) lignocellulosic feedstocks was demonstrated using the Pleurotus ostreatus enzyme heterologously expressed in Escherichia coli and "in vitro" folded. Two-dimensional nuclear magnetic resonance (NMR) of the treated materials, swollen in dimethylsulfoxide-d6, revealed a reduction in the number of lignin side-chain linkages after both treatments. However, a significant removal of lignin by the enzymatic treatment was only observed in the case of eucalypt wood, as shown by the decrease of lignin aromatic signals referred to carbohydrate anomeric signals.

Keywords: white rot; brown rot

Publication Review Process: Non-Refereed (Other)

File size: 1,718 kb(s)

Date posted: 10/01/2014
RITS Product ID: 70114
Current FPL Scientist associated with this product
Hammel, Kenneth E.
Research Chemist

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