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Title: Lignosulfonate-mediated cellulase adsorption: enhanced enzymatic saccharification of lignocellulose through weakening nonproductive binding to lignin

Source: Biotechnology for Biofuels Volume 6, 2013; 10 p.

Author(s)Wang, Zhaojiang; Zhu, JY; Fu, Yingjuan; Qin, Menghua; Shao, Zhiyong; Jiang, Jungang; Yang, Fang

Publication Year: 2013  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4709-1B

Abstract: Thermochemical pretreatment of lignocellulose is crucial to bioconversion in the fields of biorefinery and biofuels. However, the enzyme inhibitors in pretreatment hydrolysate make solid substrate washing and hydrolysate detoxification indispensable prior to enzymatic hydrolysis. Sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) is a relatively new process, but has demonstrated robust performance for sugar and biofuel production from woody biomass in terms of yield and energy efficiency. This study demonstrated the advantage of SPORL pretreatment whereby the presentation of lignosulfonate (LS) renders the hydrolysate non-inhibitory to cellulase (Cel) due to the formation of lignosulfonate-cellulase complexes (LCCs) which can mediate the Cel adsorption between lignin and cellulose, contrary to the conventional belief that pretreatment hydrolysate inhibits the enzymatic hydrolysis unless detoxified.

Keywords: Enzymatic saccharification/hydrolysis; Lignin/lignosulfonate; Nonproductive/nonspecific binding/adsorption; Polyelectrolyte-protein complexes; Pretreatment

Publication Review Process: Formally Refereed

File size: 3,162 kb(s)

Date posted: 01/02/2014

This publication is also viewable on Treesearch:  view
RITS Product ID: 66966
Current FPL Scientist associated with this product
Zhu, JunYong
Research General Engineer
  

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