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Title: Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism

Source: Environmental Microbiology, Volume 15, Number 3, 2013; pp. 956–966.

Author(s)Hunt, Christopher G.; Houtman, Carl J.; Jones, Don C.; Kitin, Peter; Korripally, Premsagar; Hammel, Kenneth E.

Publication Year: 2013  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4707-2B   FPL-4709-1B   FPL-4712-2B

Abstract: Oxidative cleavage of the recalcitrant plant polymer lignin is a crucial step in global carbon cycling, and is accomplished most efficiently by fungi that cause white rot of wood. These basidiomycetes secrete many enzymes and metabolites with proposed ligninolytic roles, and it is not clear whether all of these agents are physiologically important during attack on natural lignocellulosic substrates. One new approach to this problem is to infer properties of ligninolytic oxidants from their spatial distribution relative to the fungus on the lignocellulose.We grew Phanerochaete chrysosporium on wood sections in the presence of oxidant-sensing beads based on the ratiometric fluorescent dye BODIPY 581/591. The beads, having fixed locations relative to the fungal hyphae, enabled spatial mapping of cumulative extracellular oxidant distributions by confocal fluorescence microscopy. The results showed that oxidation gradients occurred around the hyphae, and data analysis using a mathematical reaction–diffusion model indicated that the dominant oxidant during incipient white rot had a half-life under 0.1 s. The best available hypothesis is that this oxidant is the cation radical of the secreted P. chrysosporium metabolite veratryl alcohol.

Keywords: Wood decay; white rot; carbon cycle; phanerochaete chrysosporium

Publication Review Process: Formally Refereed

File size: 748 kb(s)

Date posted: 05/14/2013

This publication is also viewable on Treesearch:  view
RITS Product ID: 63852
Current FPL Scientists associated with this product (listed alphabetically)
Hammel, Kenneth E.
Research Chemist
Houtman, Carl J.
Chemical Engineer
Hunt, Christopher G.
Research Chemist

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