Banner for LabNotes
From Lab Notes
Contact Information
Forest Products Laboratory
One Gifford Pinchot Drive
Madison, WI 53726-2398
Phone: (608) 231-9200
Fax: (608) 231-9592


You are here: FPL Home  / Information Products & Services  / Publications

Requested Product

Title: Pseudomonas sax Genes Overcome Aliphatic Isothiocyanate - Mediated Non-Host Resistance in Arabidopsis

Source: Science. 331: 1185 -1188. 2011

Author(s)Fan, Jun; Crooks, Casey; Creissen, Gary; Hill, Lionel; Fairhurst, Shirley; Doerner, Peter; Lamb, Chris

Publication Year: 2011  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4712-3A

Abstract: Most plant-microbe interactions do not result in disease; natural products restrict non-host pathogens. We found that sulforaphane (4-methylsulfinylbutyl isothiocyanate), a natural product derived from aliphatic glucosinolates, inhibits growth in Arabidopsis of non-host Pseudomonas bacteria in planta. Multiple sax genes (saxCAB/F/D/G) were identified in Pseudomonas species virulent on Arabidopsis. These sax genes are required to overwhelm isothiocyanate-based defenses and facilitate a disease outcome, especially in the young leaves critical for plant survival. Introduction of saxCAB genes into non-host strains enabled them to overcome these Arabidopsis defenses. Our study shows that aliphatic isothiocyanates, previously shown to limit damage by herbivores, are also crucial, robust, and developmentally regulated defenses that underpin non-host resistance in the Arabidopsis-Pseudomonas pathosystem.

Keywords: Phytopathogenic bacteria; bacterial diseases of plants; pathogenic bacteria; disease susceptibility; Arabidopsis; biodegradation; bacteria; microorganisms; molecular genetics; isothiocyanates; plants; disease resistance; pest resistance; plant chemical defenses; Pseudomonas sax; resistance to decay; phytopathogenic bacteria; biocides; aliphatic isothiocyanate; sulforaphane; genetic analysis

Publication Review Process: Formally Refereed

File size: 209 kb(s)

Date posted: 11/25/2011

This publication is also viewable on Treesearch:  view
RITS Product ID: 40269
Current FPL Scientist associated with this product
Crooks, M.E. Casey
Research Microbiologist

Additional items that might interest you
View the video celebrating FPL's 100 years of public service in 2010, from the producers of the Greatest Good....view

Research Highlights from FPL....view

Termite Eradication: A search for the Holy Grail.... view

Moisture Management in Residential Construction Series videos...view

Wood Floor Systems in Residential Construction Series videos....view
- FPL's Mission and Strategic Plan -

FPL's mission is to identify and conduct innovative wood and fiber utilization research that contributes to conservation and productivity of the forest resource, thereby sustaining forests, the economy, and quality of life. ... ..more »

- FPL Research Emphasis Areas -
Advanced Composites

As an integral part of the FPL mission, we improve the long-term sustainability of our Nation's forests by creating valuable composite products from biobased materials ... ..more »

Advanced Structures

The FPL has been in the forefront of wood-frame housing research since 1910 and has long been recognized as a world leader in such housing-related areas as engineered wood ... ..more »

Forest Biorefinery

We all know the compelling reasons that the United States needs to reduce its dependence on fossil fuels. Historically, the greatest increases in energy demand have been for transportation fuels ... ..more »


A leader in wood products research for over a century, the FPL is positioning itself to become the lead Federal research facility for the application of nanotechnology in forest products ... more »

Woody Biomass Utilization

Forests in the United States contain a substantial amount of small-diameter, overstocked, and underutilized material.FPL research projects are exploring the potential of the small-diameter ... ..more »