Banner for LabNotes
From Lab Notes
Contact Information
Forest Products Laboratory
One Gifford Pinchot Drive
Madison, WI 53726-2398
Phone: (608) 231-9200
Fax: (608) 231-9592


You are here: FPL Home  / Information Products & Services  / Publications

Requested Product

Title: High titer ethanol production from simultaneous enzymatic saccharification and fermentation of aspen at high solids: A comparison between SPORL and dilute acid pretreatments

Source: Bioresource Technology, 102 (2011) 8921-8929. doi:10.1016/j.biortech.2011.07.047; 2011

Author(s)Zhu, J.Y.; Gleisner, R.; Scott, C.T.; Luo, X.L.; Tian, S.

Publication Year: 2011  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4709-1A

Abstract: Native aspen (Populus tremuloides) was pretreated using sulfuric acid and sodium bisulfite (SPORL) and dilute sulfuric acid alone (DA). Simultaneous enzymatic saccharification and fermentation (SSF) was conducted at 18% solids using commercial enzymes with cellulase loadings ranging from 6 to 15 FPU/g glucan and Saccharomyces cerevisiae Y5. Compared with DA pretreatment, the SPORL pretreatment reduced the energy required for wood chip size-reduction, and reduced mixing energy of the resultant substrate for solid liquefaction. Approximately 60% more ethanol was produced from the solid SPORL substrate (211 L/ton wood at 59 g/L with SSF efficiency of 76%) than from the solid DA substrate (133 L/ton wood at 35 g/L with SSF efficiency 47%) at a cellulase loading of 10 FPU/g glucan after 120 h. When the cellulase loading was increased to 15 FPU/g glucan on the DA substrate, the ethanol yield still remained lower than the SPORL substrate at 10 FPU/g glucan

Keywords: Sugars, biomass energy, aspen, ethanol, biomass utilization, pretreatment, lignocellulose, biotechnology, wood chips, enzymes, industrial applications, fuelwood, fermentation, cellulose, lignin, biodegradation, fungi, yeast fungi, Populus tremuloides, Saccharomyces cerevisiae, sulfuric acid, cellulase, glucans, SPORL, biomass fuel, saccharification, bioconversion, biorefining, alcohol, chips, chemical utilization, sodium bisulfite, biofuels, high solids, simultaneous saccharification and fermentation (SSF), cellulosic ethanol titer, woody biomass

Publication Review Process: Formally Refereed

File size: 976 kb(s)

Date posted: 11/16/2011

This publication is also viewable on Treesearch:  view
RITS Product ID: 41243
Current FPL Scientist associated with this product
Zhu, JunYong
Research General Engineer

Additional items that might interest you
View the video celebrating FPL's 100 years of public service in 2010, from the producers of the Greatest Good....view

Research Highlights from FPL....view

Termite Eradication: A search for the Holy Grail.... view

Moisture Management in Residential Construction Series videos...view

Wood Floor Systems in Residential Construction Series videos....view
- FPL's Mission and Strategic Plan -

FPL's mission is to identify and conduct innovative wood and fiber utilization research that contributes to conservation and productivity of the forest resource, thereby sustaining forests, the economy, and quality of life. ... ..more »

- FPL Research Emphasis Areas -
Advanced Composites

As an integral part of the FPL mission, we improve the long-term sustainability of our Nation's forests by creating valuable composite products from biobased materials ... ..more »

Advanced Structures

The FPL has been in the forefront of wood-frame housing research since 1910 and has long been recognized as a world leader in such housing-related areas as engineered wood ... ..more »

Forest Biorefinery

We all know the compelling reasons that the United States needs to reduce its dependence on fossil fuels. Historically, the greatest increases in energy demand have been for transportation fuels ... ..more »


A leader in wood products research for over a century, the FPL is positioning itself to become the lead Federal research facility for the application of nanotechnology in forest products ... more »

Woody Biomass Utilization

Forests in the United States contain a substantial amount of small-diameter, overstocked, and underutilized material.FPL research projects are exploring the potential of the small-diameter ... ..more »