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Title: Consolidation of glycosyl hydrolase family 30: A dual domain 4/7 hydrolase family consisting of two structurally distinct groups

Source: FEBS Letters, 584 (2010) 4435-4441. doi:10.1016/j.febslet.2010.09.051; 2010

Author(s)St John, Franz J.; Gonzalez, Javier M.; Pozharski, Edwin

Publication Year: 2010  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4712-3A

Abstract: In this work glycosyl hydrolase (GH) family 30 (GH30) is analyzed and shown to consist of its currently classified member sequences as well as several homologous sequence groups currently assigned within family GH5. A large scale amino acid sequence alignment and a phylogenetic tree were generated and GH30 groups and subgroups were designated. A partial rearrangement in the GH30 defining side-associated β-domain contributes to the differentiation of two major groups that contain up to eight subgroups. For this CAZy family of Clan A enzymes the dual domain fold is conserved, suggesting that it may be a requirement for evolved function. This work redefines GH family 30 and serves as a guide for future efforts regarding enzymes classified within this family.

Keywords: Enzymes, nucleotide sequence, phylogeny, molecular genetics, amino acids, proteins, carbohydrates, catalysis, hydrolases, glycosyl hydrolase, genetic analysis, glucuromoxylan xylanohydrolase, endo-[beta]-1,6-galactanase, endo-[beta]-1,6-glucanase, glucosylceramidase

Publication Review Process: Formally Refereed

File size: 696 kb(s)

Date posted: 11/16/2011

This publication is also viewable on Treesearch:  view
RITS Product ID: 40271
Current FPL Scientist associated with this product
St. Johns, Franz
Research Microbiologist

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