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Title: Multidimensional NMR analysis reveals truncated lignin structures in wood decayed by the brown rot basidiomycete Postia placenta

Source: Environmental Microbiology (2011). Volume 13, Number 4, 1091-1100; 2011

Author(s)Yelle, Daniel J.; Wei, Dongsheng; Ralph, John; Hammel, Kenneth E.

Publication Year: 2011  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4707-3A  FPL-4712-2A

Abstract: Lignocellulose biodegradation, an essential step in terrestrial carbon cycling, generally involves removal of the recalcitrant lignin barrier that otherwise prevents infiltration by microbial polysaccharide hydrolases. However, fungi that cause brown rot of wood, a major route for biomass recycling in coniferous forests, utilize wood polysaccharides efficiently while removing little of the lignin. The mechanism by which these basidiomycetes breach the lignin remains unclear. We used recently developed methods for solubilization and multidimensional 1H-13C solution-state NMR spectroscopy of ball-milled lignocellulose to analyse aspen wood degraded by Postia placenta. The results showed that decay decreased the content of the principal arylglycerol-β-aryl ether interunit linkage in the lignin by more than half, while increasing the frequency of several truncated lignin structures roughly fourfold over the level found in sound aspen. These new end-groups, consisting of benzaldehydes, benzoic acids and phenylglycerols, accounted for 6-7% of all original lignin subunits. Our results provide evidence that brown rot by P. placenta results in significant ligninolysis, which might enable infiltration of the wood by polysaccharide hydrolases even though the partially degraded lignin remains in situ. Recent work has revealed that the P. placenta genome encodes no ligninolytic peroxidases, but has also shown that this fungus produces an extracellular Fenton system. It is accordingly likely that P. placenta employs electrophilic reactive oxygen species such as hydroxyl radicals to disrupt lignin in wood.

Keywords: Wood-decaying fungi, brown rot, lignocellulose, biodegradation, Basidiomycetes, lignin, aspen, microbial metabolism, Postia placenta, polysaccharides, nuclear magnetic resonance spectroscopy, Populus, benzaldehyde, benzoic acid, hydrolases, wood deterioration, decay fungi, wood decay, phenylglycerol, ligninolysis, Fenton system, hydroxyl radicals, peroxidase

Publication Review Process: Formally Refereed

File size: 1 kb(s)

Date posted: 11/07/2011

This publication is also viewable on Treesearch:  view
RITS Product ID: 40178
Current FPL Scientists associated with this product (listed alphabetically)
Hammel, Kenneth E.
Research Chemist
Yelle, Daniel
Research Forest Products Technologist
 

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