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Title: A rapid colorimetric assay for mold spore germination using XTT tetrazolium salt

Source: In: IRG/WP 11-20462 THE INTERNATIONAL RESEARCH GROUP ON WOOD PROTECTION, Paper prepared for 42nd Annual Meeting Queenstown, New Zealand 8-12 May, 2011, Section 2, Test Methodology and Assessment; 2011

Author(s)Clausen, Carol A.; Yang, Vina W.

Publication Year: 2011  View PDF »

Category: Conference Proceedings

Abstract: Current laboratory test methods to measure efficacy of new mold inhibitors are time consuming, some require specialized test equipment and ratings are subjective. Rapid, simple quantitative assays to measure the efficacy of mold inhibitors are needed. A quantitative, colorimetric microassay was developed using XTT tetrazolium salt to metabolically assess mold spore germination. Quantitative values for the inhibition of mold spore germination using the XTT assay were 30-fold lower for thiabendazole and Durazol than the calculated MIC90 based on subjective ratings using current standard methods. The XTT assay can be used to demonstrate the relative resistance of different fungal species to antifungal agents, to compare the relative inhibition of a new antifungal agent to existing antifungal agents, or to screen new wood preservative formulations for their susceptibility to mold growth.

Keywords: Biodegradation; wood-decaying fungi; mold control; biodegradation; fungicides; antifungal agents; salts; tetrazolium salts; colorimetry; spores; susceptibility; wood preservatives; biocides; resistance to decay; decay fungi; wood decay; XTT assay; thiabendazole; Durazol; preservative treated wood; treated wood; antifungal; colorimetric assay; mold fungi; spore germination

Publication Review Process: Informally Refereed (Peer-Reviewed)

File size: 524 kb(s)

Date posted: 06/06/2011

This publication is also viewable on Treesearch:  view
RITS Product ID: 38984
Current FPL Scientist associated with this product
Clausen, Carol A.
Supervisory Research Microbiologist

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