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Title: The Usual Suspects: Fingerprinting Microbial Communities Involved in Decay of Treated Southern Yellow Pine

Source: In: Proceedings of the 106 annual meeting of the American Wood Protection Association, Hyatt Regency Riverfront, Savannah, Georgia May 23-25, 2010, Volume 106, pp. 127-133; 2010

Author(s)Kirker, Grant T.; Diehl, Susan V.; Prewitt, M. Lynn

Publication Year: 2010  View PDF »

Category: Conference Proceedings
Associated Research Project(s):   FPL-4723-3

Abstract: Current standards for soil-block testing have long been based on the effectiveness of preservative systems against only a small number of wood decay fungi and even fewer bacteria. Culture-independent molecular methods offer simple, reproducible means to obtain a more holistic view of the microbial communities that colonize wood throughout the decay process. By using a culture-independent PCR-based method called terminal restriction fragment length polymorphism (T-RFLP) analysis, we were able to detect shifts of fungal and bacterial communities in wood treated with sub-lethal concentrations of ACQ-C and CTN. T-RFLP takes into account all species of a taxonomic group and creates a community profile or 'fingerprint,' where each peak in the profile represents a unique species. Both compounds appeared to change the patterns of bacterial succession completely, so that beginning and ending communities were significantly different in regard to species composition. Fungal species community structure was initially changed, but became more similar to untreated controls over time, presumably as the preservatives were depleted from samples. Subsequent depletion analysis found >60% depletion of preservatives from treated field stakes after 15 months exposure. Further modification to this process will eventually enable us to accurately identify fungal and bacterial species making up the microbial communities found in treated and decaying wood and offer new insights into the decay process.

Keywords: Biodegradation; wood deterioration; decay prevention; wood-decaying fungi; wood biodegradation; fungicides; wood preservatives; preserved wood; preservative testing; bacteria; antifungal agents; copper; fungal communities; microorganisms; microbial communities; BHT; preservatives; Butylated hydroxytoluene; BHT; chlorothalonil; field tests; exposure tests; decay fungi; wood decay; southern yellow pine; preservation; decay resistance; preservative treated wood; treated wood; biocides; ACQ; alkaline copper quaternary; wood decomposition; fungicidal properties; copper compounds; CTN; T-RFLP; stake tests; stake decay; microbial community analysis

Publication Review Process: Informally Refereed (Peer-Reviewed)

File size: 196 kb(s)

Date posted: 03/08/2011

This publication is also viewable on Treesearch:  view
RITS Product ID: 37872
Current FPL Scientist associated with this product
Kirker, Grant
Research Forest Products Technologist

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