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Title: [34] Fluorenscent Labels, Confocal Microscopy, and Quantitative Image Analysis in Study of Fungal Biology

Source: METHODS IN ENZYMOLOGY, VOL. 307; pp. 607-623

Author(s)Spear, Russell; Cullen, Daniel; Andrews, John;

Publication Year: 1999  View PDF »

Category: Journal Articles

Abstract: Microscopy in the study of fungi has been applied basically in two contexts. First, the fungus in isolation can be the subject of investigation, in which case studies typically have focused on cell dynamics, morphogenesis, cytochemistry, and organelle or spore structure and development. Within the past decade, laser scanning confocal microscopy (LSCM) has become a powerful tool in the analysis of subcellular detail such as that just noted.1-4 It combines laser, computer, and imaging technologies to enable optical rather than mechanical sectioning of specimens. The second context pertains to the relationship between a fungus and its substratum. Examples include host-fungus interactions and the extent or spatial pattern of colonization. LSCM can reconstruct multiple, in-focus image planes, rendering them in three dimensions. Thus, it has been used to obtain sharp images of fungi within a host or colonizing highly contoured or rugged surfaces such as the phylloplane or rhizoplane of plants.

File size: 293 kb(s)

Date posted: 07/19/2010
Current FPL Scientist associated with this product
Cullen, Daniel
Research Microbiologist
  

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