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Title: Comparative Transcriptome and Secretome Analysis of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium

Source: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2010, p. 3599-3610; 2010

Author(s)Wymelenberg, Amber Vanden; Gaskell, Jill; Mozuch, Michael; Sabat, Grzegorz; Ralph John; Skyba, Oleksandr; Mansfield, Shawn D.; Blanchette, Robert A.; Martinez, Diego; Grigoriev, Igor; Kersten, Philip J.; Cullen, Dan

Publication Year: 2010  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4712-4A

Abstract: Cellulose degradation by brown rot fungi, such as Postia placenta, is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium. To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi cultured for 5 days in media containing ball-milled aspen or glucose as the sole carbon source. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), a total of 67 and 79 proteins were identified in the extracellular fluids of P. placenta and P. chrysosporium cultures, respectively. Viewed together with transcript profiles, P. chrysosporium employs an array of extracellular glycosyl hydrolases to simultaneously attack cellulose and hemicelluloses. In contrast, under these same conditions, P. placenta secretes an array of hemicellulases but few potential cellulases. The two species display distinct expression patterns for oxidoreductase-encoding genes. In P. placenta, these patterns are consistent with an extracellular Fenton system and include the upregulation of genes involved in iron acquisition, in the synthesis of low-molecular-weight quinones, and possibly in redox cycling reactions.

Keywords: Liquid chromatography; cellulose; biodegradation; mass spectrometry; enzymes; biotechnology; lignocellulose; Basidiomycetes; molecular genetics; fungi genetics; fungi; lignin; biodegradation; wood-decaying fungi; industrial applications; gene expression; genetic transcription; Postia placenta; Phanerochaete chrysosporium; brown rot; aspen; glucose; iron; quinone; chemical reactions; Populus; transcriptome; wood decay; secretome; white rot; decay fungi; Fenton system

Publication Review Process: Formally Refereed

File size: 1,242 kb(s)

Date posted: 07/15/2010

This publication is also viewable on Treesearch:  view
RITS Product ID: 35563
Current FPL Scientists associated with this product (listed alphabetically)
Cullen, Daniel
Research Microbiologist
Kersten, Philip J.
Research Microbiologist

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