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Title: Selection and Improvement of Lignin-Degrading Microorganisms: Potential Strategy Based on Lignin Model-Amino Acid Adducts

Source: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 1987, p. 242-245; 1987

Author(s)Tien, Ming; Kersten, Philip J.; Kirk, T. Kent

Publication Year: 1987  View PDF »

Category: Journal Articles

Abstract: The purpose of this investigation was to test a potential strategy for the ligninase-dependent selection of lignin-degrading microorganisms. The strategy involves covalently bonding amino acids to lignin model compounds in such a way that ligninase-catalyzed cleavage of the models releases the amino acids for growth nitrogen. Here we describe the synthesis of glycine-N-2-(3,4-dimethoxyphenyl)ethane-2-ol (I) and demonstrate that growth (as measured by mycelial nitrogen content) of the known lignin-degrading basidiomycete Phanerochaete chrysosporium Burds. with compound I as the nitrogen source depends on its production of ligninase. Ligninase is shown to catalyze the oxidative C-C cleavage of compound I, releasing glycine, formaldehyde,andveratraldehyde ata 1:1:1 stoichiometry.P. chrysosporium utilizes compound I as a nitrogen source, but only after the cultures enter secondary metabolism (day 3 of growth), at which time the ligninase and the other components of the ligninolytic system (lignin -> C02) are expressed. Compound I and related adducts have potential not only in the isolation of lignin-degrading microbes but, perhaps of equal importance, in strain improvement.

Keywords: lignin peroxidase, wood decay, Phanerochaete chrysosporium, selection, ligninase

File size: 751 kb(s)

Date posted: 05/19/2010
Current FPL Scientist associated with this product
Kersten, Philip J.
Research Microbiologist
  

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