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Title: Involvement of a New Enzyme, Glyoxal Oxidase, in Extracellular H2O2 Production by Phabnerochaete chrysosporium

Source: JOURNAL OF BACTERIOLOGY, May 1987, Vol. 169, No. 5, p. 2195-2201; 1987

Author(s)Kersten, Philip J.; Kirk, T. Kent

Publication Year: 1987  View PDF »

Category: Journal Articles

Abstract: The importance of extracellular H2O2 in lignin degradation has become increasingly apparent with the recent discovery of H2O2-requiring ligninases produced by white-rot fungi. Here we describe a new H2O2-producing activity of Phanerochaete chrysosporium that involves extracellular oxidases able to use simple aldehyde, a-hydroxycarbonyl, or a-dicarbonyl compounds as substrates. The activity is expressed during secondary metabolism, when the ligninases are also expressed. Analytical isoelectric focusing of the extracellular proteins, followed by activity staining, indicated that minor proteins with broad substrate specificities are responsible for the oxidase activity. Two of the oxidase substrates, glyoxal and methylglyoxal, were also identified, as their quinoxaline derivatives, in the culture fluid as secondary metabolites. The significance of these findings is discussed with respect to lignin degradation and other proposed systems for H2O2 production in P. chrysosporium.

Keywords: Phanerochaete chrysosporium, Glyoxal Oxidase

File size: 580 kb(s)

Date posted: 05/19/2010
Current FPL Scientist associated with this product
Kersten, Philip J.
Research Microbiologist

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