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Title: Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium

Source: Nucleic Acids Research, Vol. 19, No. 3; pp. 599-603; 1991

Author(s)Gaskell, Jill; Dieperink, Eric; Cullen, Daniel

Publication Year: 1991  View PDF »

Category: Journal Articles

Abstract: Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmld insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresls (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.

Keywords: Lignin peroxidase; Phanerochaete chrysosporium; basidiomycete; genomic organization; lignolytic

File size: 3,693 kb(s)

Date posted: 05/19/2010
Current FPL Scientist associated with this product
Cullen, Daniel
Research Microbiologist
  

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