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Title: Isolation and characterization of homokaryotic strains from the ligninolytic baqsidiomycete Cerioporiopsis subvermispora

Source: FEMS Microbiology Letters 199 (2001) 91-96

Author(s)Tello, Seelenfreund, Lobos, Gaskell, Cullen, Vicuna

Publication Year: 2001  View PDF »

Category: Journal Articles

Abstract: Genetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of spore forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB, their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of new genes. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

Keywords: Homokaryon ; Basidiomycete; Manganese peroxidase; Ligninolytic; Ceriporiopsis subvermispora

File size: 277 kb(s)

Date posted: 04/05/2010
Current FPL Scientist associated with this product
Cullen, Daniel
Research Microbiologist

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