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Title: Structure, Organization, and Transcription of a Cellobiohydrolase Gene Cluster from Phanerochaete chrysosporium

Source: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, July 1992, p. 2168-2175; 1992

Author(s)Covert, Sarah F.; Wymelenberg, Amber Vanden; Cullen, Daniel

Publication Year: 1992  View PDF »

Category: Journal Articles

Abstract: Restriction mapping and sequence analysis of cosmid clones revealed a cluster of three cellobiohydrolase genes in Phanerochaete chrysosporium. P. chrysosporium cbh1-1 and cbh1-2 are separated by only 750 bp and are located approximately 14 kb upstream from a cellulase gene previously cloned from P. chrysosporium (P. Sims, C. James, and P. Broda, Gene 74:411422, 1988). Within a well-conserved region, the deduced amino acid sequences of P. chrysosporium cbh1-1 and cbh1-2 are, respectively, 80 and 69% homologous to that of the Trichoderma reesei cellobiohydrolase I gene. The conserved cellulose-binding domain typical of microbial cellulases is absent from cbh1-1. Transcript levels of the three P. chrysosporium genes varied substantially, depending on culture conditions. cbh1-1 and cbh1-2 were not induced in the presence of cellulose, nor did they appear to be subject to glucose repression. Therefore, aspects of the chromosomal organization, structure, and transcription of these genes are unlike those of any previously described cellulase genes.

Keywords: Structure, organization, transcription, Cellobiohydrolase gene, Phanerochaete chrysosporium

File size: 510 kb(s)

Date posted: 02/09/2010
Current FPL Scientist associated with this product
Cullen, Daniel
Research Microbiologist
  

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