Banner for LabNotes
From Lab Notes
Contact Information
Forest Products Laboratory
One Gifford Pinchot Drive
Madison, WI 53726-2398
Phone: (608) 231-9200
Fax: (608) 231-9592


You are here: FPL Home  / Information Products & Services  / Publications

Requested Product

Title: Genomic organization of a cellulase gene familyin Phanerochaete chrysosporium

Source: Curr Genet (1992) 22:407-413

Author(s)Covert, Sarah F.; Bolduc, Jennifer; Cullen, Dan

Publication Year: 1992  View PDF »

Category: Journal Articles

Abstract: Southern blot and nucleotide sequence analysis of Phanerochaete chrysosporium BKM-F-1767 genomic clones indicate that this wood-degrading fungus contains at least six genes with significant homology to the Trichoderma reesei cellobiohydrolase I gene (cbh1). Using pulsed-field gel electrophoresis to separate P. chrysosporium chromosomes, the six cellulase genes were found to hybridize to at least three different chromosomes, one of which is dimorphic. The organization of these genes was similar in another P. chrysosporium strain, ME 446. It is clear that, unlike T. reesei, the most well-studied cellulolytic fungus, P. chrysosporium contains a complex, cbh1-like gene family.

Keywords: Molecular genetics; fungi; genetics; wood-decaying fungi; biodegradation; fungi; biotechnology; Basidiomycetes; nucleotide sequence; gene mapping; cellulase; genomes; cellulose 1;4-beta-cellobiosidase; lignocellulose; gel electrophoresis; Phanerochaete chrysosporium; genetic engineering; wood decay; white rot; Trichoderma reesei; pulsed-field gel electrophoresis; southern blot analysis

Publication Review Process: Informally Refereed (Peer-Reviewed)

File size: 3,576 kb(s)

Date posted: 02/09/2010

This publication is also viewable on Treesearch:  view
RITS Product ID: 35694
Current FPL Scientist associated with this product
Cullen, Daniel
Research Microbiologist

Additional items that might interest you
View the video celebrating FPL's 100 years of public service in 2010, from the producers of the Greatest Good....view

Research Highlights from FPL....view

Termite Eradication: A search for the Holy Grail.... view

Moisture Management in Residential Construction Series videos...view

Wood Floor Systems in Residential Construction Series videos....view
- FPL's Mission and Strategic Plan -

FPL's mission is to identify and conduct innovative wood and fiber utilization research that contributes to conservation and productivity of the forest resource, thereby sustaining forests, the economy, and quality of life. ... ..more »

- FPL Research Emphasis Areas -
Advanced Composites

As an integral part of the FPL mission, we improve the long-term sustainability of our Nation's forests by creating valuable composite products from biobased materials ... ..more »

Advanced Structures

The FPL has been in the forefront of wood-frame housing research since 1910 and has long been recognized as a world leader in such housing-related areas as engineered wood ... ..more »

Forest Biorefinery

We all know the compelling reasons that the United States needs to reduce its dependence on fossil fuels. Historically, the greatest increases in energy demand have been for transportation fuels ... ..more »


A leader in wood products research for over a century, the FPL is positioning itself to become the lead Federal research facility for the application of nanotechnology in forest products ... more »

Woody Biomass Utilization

Forests in the United States contain a substantial amount of small-diameter, overstocked, and underutilized material.FPL research projects are exploring the potential of the small-diameter ... ..more »