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Title: Genomic organization of a cellulase gene familyin Phanerochaete chrysosporium

Source: Curr Genet (1992) 22:407-413

Author(s)Covert, Sarah F.; Bolduc, Jennifer; Cullen, Dan

Publication Year: 1992  View PDF »

Category: Journal Articles

Abstract: Southern blot and nucleotide sequence analysis of Phanerochaete chrysosporium BKM-F-1767 genomic clones indicate that this wood-degrading fungus contains at least six genes with significant homology to the Trichoderma reesei cellobiohydrolase I gene (cbh1). Using pulsed-field gel electrophoresis to separate P. chrysosporium chromosomes, the six cellulase genes were found to hybridize to at least three different chromosomes, one of which is dimorphic. The organization of these genes was similar in another P. chrysosporium strain, ME 446. It is clear that, unlike T. reesei, the most well-studied cellulolytic fungus, P. chrysosporium contains a complex, cbh1-like gene family.

Keywords: Molecular genetics, fungi, genetics, wood-decaying fungi, biodegradation, fungi, biotechnology, Basidiomycetes, nucleotide sequence, gene mapping, cellulase, genomes, cellulose 1,4-beta-cellobiosidase, lignocellulose, gel electrophoresis, Phanerochaete chrysosporium, genetic engineering, wood decay, white rot, Trichoderma reesei, pulsed-field gel electrophoresis, southern blot analysis

Publication Review Process: Informally Refereed (Peer-Reviewed)

File size: 3,576 kb(s)

Date posted: 02/09/2010

This publication is also viewable on Treesearch:  view
RITS Product ID: 35694
Current FPL Scientist associated with this product
Cullen, Daniel
Research Microbiologist
  

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