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Title: Sequence and centromere proximal location of a transformation enhancing fragment ans1 from Aspergillus nidulans

Source: Nucleic Acids Research, Vol 15, Number 22, pp. 9163-9175; 1987

Author(s)Cullen, D.; Wilson, L.J.; Grey, G.L.; Henner, D.J.; Turner, G.; Balance, D.J.

Publication Year: 1987  View PDF »

Category: Journal Articles

Abstract: The Asperqillus nidulans sequence ans1, previously known to enhance transformation frequencies of pyr4-based vectors, was shown to enhance the efficiency of argB and trpC-based vectors. Increased efficiencies could be obtained by constructing vectors containing argB and ans1 or by cotransforming selectable plasmids (containing argB, trpC or pyr4) with the non-selectable ans1 sequence. The preponderance of evidence suggests that the mechanism of ans1 activity does not involve homologous recombination events, in spite of the presence of multiple regions of homology in the A. nidulans genome. Genetic mapping localized ans1 to the vicinity of the centromere of linkage group I. The nucleotide sequence of a 1.8 Kb functional subclone of ans1 was determined and found to be highly A+T rich (81%).

Keywords: ans1, Aspergillus nidulans, centromere proximal location, Sequence

File size: 1,059 kb(s)

Date posted: 02/09/2010
Current FPL Scientist associated with this product
Cullen, Daniel
Research Microbiologist
  

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