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Title: Identification of Catalytic Residues in Glyoxal Oxidase by Targeted Mutagenesis*

Source: J. BIOLOGICAL CHEMISTRY. Vol. 274, No. 61, pp. 36226-36232, 1999

Author(s)Whittaker, M., Kersten, Cullens, Whittaker, J.

Publication Year: 1999  View PDF »

Category: Journal Articles

Abstract: Glyoxal oxidase is a copper metalloenzyme produced by the wood-rot fungus Phanerochaete chrysosporium as an essential component of its extracellular lignin degradation pathways. Previous spectroscopic studies on glyoxal oxidase have demonstrated that it contains a free radical-coupled copper active site remarkably similar to that found in another fungal metalloenzyme, galactose oxidase. Alignment of primary structures has allowed four catalytic residues of glyoxal oxidase to be targeted for site-directed mutagenesis in the recombinant protein. Three glyoxal oxidase mutants have been heterologously expressed in both a filamentous fungus (Aspergillus nidulans) and in a methylotrophic yeast (Pichia pastoris), the latter expression system producing as much as 2 g of protein per liter of culture medium under conditions of high density methanol-induced fermentation. Biochemical and spectroscopic characterization of the mutant enzymes supports structural correlations between galactose oxidase and glyoxal oxidase, clearly identifying the catalytically important residues in glyoxal oxidase and demonstrating the functions of each of these residues.

File size: 326 kb(s)

Date posted: 10/19/2009
Current FPL Scientists associated with this product (listed alphabetically)
Cullen, Daniel
Research Microbiologist
Kersten, Philip J.
Research Microbiologist
 

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