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Title: Sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) for robust enzymatic saccharification of hardwoods

Source: Biotechnol. Prog., 2009, Vol. 25, No. 4; pp. 1086 - 1093; 2009

Author(s)Wang, G.S.; Pan, X.J.; Zhu, J.Y.; Gleisner, R.; Rockwood, D.

Publication Year: 2009  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4709-1A

Abstract: This study demonstrates sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) for robust bioconversion of hardwoods. With only about 4% sodium bisulfite charge on aspen and 30-min pretreatment at temperature 180C, SPORL can achieve near-complete cellulose conversion to glucose in a wide range of pretreatment liquor of pH 2.0 - 4.5 in only about 10 h enzymatic hydrolysis. The enzyme loading was about 20 FPU cellulase plus 30 CBU b-glucosidase per gram of cellulose. The production of fermentation inhibitor furfural was less than 20 mg/g of aspen wood at pH 4.5. With pH 4.5, SPORL avoided reactor corrosion problem and eliminated the need for substrate neutralization prior to enzymatic hydrolysis. Similar results were obtained from maple and eucalyptus.

Keywords: Sulfite, bisulfite, pretreatment, enzymatic hydrolysis, recalcitrance, lignocellulose, saccharification, hardwood, biomass, fermentation inhibitors, size reduction, SPORL, enzymes, biotechnology, industrial applications, biodegradation, cellulose, hydrolysis, glucose, fermentation, biomass energy, energy consumption, sugars, wood chips, aspen, maple, eucalyptus, cellulase, feedstock, populus, bioconversion, chemical utilization, biorefining, wood extractives, chips, Acer, sodium bisulfite, sulfite liquors, beta glucosidase

File size: 648 kb(s)

Date posted: 10/06/2009

This publication is also viewable on Treesearch:  view
RITS Product ID: 32209
Current FPL Scientist associated with this product
Zhu, JunYong
Research General Engineer

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