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Title: Structure, organization, and transcriptional regulation of a family of copper radical oxidase genes in the lignin-degrading basidiomycete Phanerochaete chrysosporium

Source: Applied and environmental microbiology. Vol. 72, no. 7 (July 2006): p. 4871-4877.

Author(s)Vanden Wymelenberg, Amber; Sabat, Grzegorz; Mozuch, Michael; Kersten, Philip J.; Cullen, Dan; Blanchette, Robert A.

Publication Year: 2006  View PDF »

Category: Journal Articles

Abstract: The white rot basidiomycete Phanerochaete chrysosporium produces an array of nonspecific extracellular enzymes thought to be involved in lignin degradation, including lignin peroxidases, manganese peroxidases, and the H2O2-generating copper radical oxidase, glyoxal oxidase (GLX). Preliminary analysis of the P. chrysosporium draft genome had identified six sequences with significant similarity to GLX and designated cro1 through cro6. The predicted mature protein sequences diverge substantially from one another, but the residues coordinating copper and constituting the radical redox site are conserved. Transcript profiles, microscopic examination, and lignin analysis of inoculated thin wood sections are consistent with differential regulation as decay advances. The cro2-encoded protein was detected by liquid chromatography-tandem mass spectrometry in defined medium. The cro2 cDNA was successfully expressed in Aspergillus nidulans under the control of the A. niger glucoamylase promoter and secretion signal. The recombinant CRO2 protein had a substantially different substrate preference than GLX. The role of structurally and functionally diverse cro genes in lignocellulose degradation remains to be established.

Keywords: Liquid chromatography, enzymes, mass spectrometry, peroxidases, Basidiomycetes, lignin, biodegradation, wood-decaying fungi, oxidases, copper, genetic transcription, Phanerochaete chrysosporium, white rot

File size: 152 kb(s)

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RITS Product ID: 11746
Current FPL Scientists associated with this product (listed alphabetically)
Cullen, Daniel
Research Microbiologist
Kersten, Philip J.
Research Microbiologist
 

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