Banner for LabNotes
From Lab Notes
Contact Information
Forest Products Laboratory
One Gifford Pinchot Drive
Madison, WI 53726-2398
Phone: (608) 231-9200
Fax: (608) 231-9592
Email

 

You are here: FPL Home  / Information Products & Services  / Publications

Requested Product

Title: Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine

Source: Bioresource Technology 100 (2009) 2411-2418; 2009

Author(s)Zhu, Pan, Wang, Gleisner

Publication Year: 2009  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4709-1A

Abstract: This study established a novel process using sulfite pretreatmet to over come recalcitrance of lignocel-lulose (SPORL) for robust and efficient bioconversion of softwoods. The process consists of sulfite treat-ment of wood chips under acidi cconditions followed by mechanical size reduction using diskrefining. The results indicated that after the SPORL pretreatment of spruce chips with 8-10% bisulfite and 1.8-3.7% sulfuric acid on ovendry(od) wood at 180C for 30 min, more than 90% cellulose conversion of substrate was achieved with enzyme loading of about 14.6 FPU cellulase plus 22.5CBU b-glucosidase per gram of odsubstrate after 48 hhydrolysis. Glucose yield from enzymatichydrolysis of the substrate per 100g of untreated odsprucewood (glucancontent43%) was about 37g (excluding the dissolved glu-coseduring pretreatment). Hemicellulose removal was found to be as critical as ligninsulfonationforcel-lulose conversion in the SPORL process. Pretreatment altered the wood chips,which reduced electric energy consumption for size reduction to about 19Wh/kg od untreated wood, or about 19g glucose/ Wh electricity. Furthermore, the SPORL produced low amounts of fermentation inhibitors, hydroxy-methyl furfural(HMF) and furfural, of about 5 and 1mg/gof untreated odwood, respectively. In addition, similar results were achieved when the SPORL was applied to redpine. By building on the mature sulfite pulping and disk refining technologies already practiced in the pulp and paper industry,the SPORL has very few technological barriers and risks for commercialization.

Keywords: Enzymatic hydrolysis, size reduction, softwood, sulfite pretreatment, SPORL, pretreatment, enzymes, biotechnology, industrial applications, lignocellulose, biodegradation, spruce, cellulose, hydrolysis, glucose, hemicellulose, fermentation, alcohol, fuelwood, biomass energy, sulfuric acid, energy consumption, furfural, red pine, wood chips, biomass utilization, sugars

File size: 300 kb(s)

This publication is also viewable on Treesearch:  view
RITS Product ID: 28637
Current FPL Scientist associated with this product
Zhu, JunYong
Research General Engineer
  

Additional items that might interest you
View the video celebrating FPL's 100 years of public service in 2010, from the producers of the Greatest Good....view

Research Highlights from FPL....view

Termite Eradication: A search for the Holy Grail.... view

Moisture Management in Residential Construction Series videos...view

Wood Floor Systems in Residential Construction Series videos....view
- FPL's Mission and Strategic Plan -

FPL's mission is to identify and conduct innovative wood and fiber utilization research that contributes to conservation and productivity of the forest resource, thereby sustaining forests, the economy, and quality of life. ... ..more »

- FPL Research Emphasis Areas -
Advanced Composites

As an integral part of the FPL mission, we improve the long-term sustainability of our Nation's forests by creating valuable composite products from biobased materials ... ..more »


Advanced Structures

The FPL has been in the forefront of wood-frame housing research since 1910 and has long been recognized as a world leader in such housing-related areas as engineered wood ... ..more »


Forest Biorefinery

We all know the compelling reasons that the United States needs to reduce its dependence on fossil fuels. Historically, the greatest increases in energy demand have been for transportation fuels ... ..more »


Nanotechnology

A leader in wood products research for over a century, the FPL is positioning itself to become the lead Federal research facility for the application of nanotechnology in forest products ... more »


Woody Biomass Utilization

Forests in the United States contain a substantial amount of small-diameter, overstocked, and underutilized material.FPL research projects are exploring the potential of the small-diameter ... ..more »