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Title: Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine

Source: Bioresource Technology 100 (2009) 2411-2418; 2009

Author(s)Zhu, Pan, Wang, Gleisner

Publication Year: 2009  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4709-1A

Abstract: This study established a novel process using sulfite pretreatmet to over come recalcitrance of lignocel-lulose (SPORL) for robust and efficient bioconversion of softwoods. The process consists of sulfite treat-ment of wood chips under acidi cconditions followed by mechanical size reduction using diskrefining. The results indicated that after the SPORL pretreatment of spruce chips with 8-10% bisulfite and 1.8-3.7% sulfuric acid on ovendry(od) wood at 180C for 30 min, more than 90% cellulose conversion of substrate was achieved with enzyme loading of about 14.6 FPU cellulase plus 22.5CBU b-glucosidase per gram of odsubstrate after 48 hhydrolysis. Glucose yield from enzymatichydrolysis of the substrate per 100g of untreated odsprucewood (glucancontent43%) was about 37g (excluding the dissolved glu-coseduring pretreatment). Hemicellulose removal was found to be as critical as ligninsulfonationforcel-lulose conversion in the SPORL process. Pretreatment altered the wood chips,which reduced electric energy consumption for size reduction to about 19Wh/kg od untreated wood, or about 19g glucose/ Wh electricity. Furthermore, the SPORL produced low amounts of fermentation inhibitors, hydroxy-methyl furfural(HMF) and furfural, of about 5 and 1mg/gof untreated odwood, respectively. In addition, similar results were achieved when the SPORL was applied to redpine. By building on the mature sulfite pulping and disk refining technologies already practiced in the pulp and paper industry,the SPORL has very few technological barriers and risks for commercialization.

Keywords: Enzymatic hydrolysis, size reduction, softwood, sulfite pretreatment, SPORL, pretreatment, enzymes, biotechnology, industrial applications, lignocellulose, biodegradation, spruce, cellulose, hydrolysis, glucose, hemicellulose, fermentation, alcohol, fuelwood, biomass energy, sulfuric acid, energy consumption, furfural, red pine, wood chips, biomass utilization, sugars

File size: 300 kb(s)

This publication is also viewable on Treesearch:  view
RITS Product ID: 28637
Current FPL Scientist associated with this product
Zhu, JunYong
Research General Engineer

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