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Title: Detection of brown-rot antigens in southern pine

Source: International Research Group on Wood Preservation : Section 2 : Test Methodology and Assessment : prepared for the 27th Annual Meeting, Guadaloupe, French West Indies, 19-24 May 1996. Stockholm, Sweden : IRG Secretariat, 1996. 8 pages

Author(s)Clausen, Carol A.

Publication Year: 1996  View PDF »

Category: Journal Articles

Abstract: Brown-rot fungal antigens were detected by particle capture immunoassay(PCI) in southern pine 2 X 4's beyond visible or culturable hyphal growth. Further analysis of test samples revealed changes along the 2 X 4's that could be grouped into zones. Zone 1, the point of inoculation through 6 cm, showed low pH, measurable oxalic acid, high moisture, and high protein. Zone 2, through 16 cm, marked the end of visible hyphal growth, increased permeability, viable fungi isolated by culturing, and detection by microscopic examination. Zone 3, through 71 cm, revealed increased alkali solubility. Zone 4, through 84 cm, demonstrated positive PCI results (i.e. detection of fungal antigens). Further testing included mineral analysis which revealed iron accumulation. Movement of soluble fungal antigens beyond hyphal growth may account for the ability of the PCI to detect the presence of brown-rot fungi at zero percent wood weight lose.

Keywords: Brown-rot fungi; particle capture immunoassay

File size: 54 kb(s)

This publication is also viewable on Treesearch:  view
RITS Product ID: 16611
Current FPL Scientist associated with this product
Clausen, Carol A.
Supervisory Research Microbiologist

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