Banner for LabNotes
From Lab Notes
Contact Information
Forest Products Laboratory
One Gifford Pinchot Drive
Madison, WI 53726-2398
Phone: (608) 231-9200
Fax: (608) 231-9592
Email

 

You are here: FPL Home  / Information Products & Services  / Publications

Requested Product

Title: Raman imaging of lignin and cellulose distribution in black spruce wood (Picea mariana) cell walls

Source: Proceedings of the 59th APPITA Annual Conference and Exhibition incorporating the 13th ISWFPC (International Symposium on Wood, Fibre, and Pulping Chemistry), held in Auckland, New Zealand (May 16-19, 2005). [Carlton, Victoria, Australia]: APPITA, c2005.

Author(s)Agarwal, Umesh P.

Publication Year: 2005  View PDF »

Category: Journal Articles

Abstract: A detailed understanding of wood cell wall structure and organization is important from both fundamental and practical point of views. A state-of- the-art 633-nm laser based confocal Raman microscope was used in situ to investigate the cell wall organization of black spruce wood. Chemical information on lignin and cellulose from morphologically distinct cell wall regions was obtained and Raman images of their distribution were generated. The initial findings were that lignin and cellulose concentration in tracheid cell walls differed significantly both within and between the distinct morphological regions. For instance, secondary wall region of some cells showed lignin concentration variation by as much as 100%. Although, normally the concentration of lignin was higher in the compound middle lamella region (CmL) compared to secondary wall (S2 and S3), there were numerous instances when the magnitudes of the two concentrations were similar or were even reversed. Some of the true cell comer middle lamella (CCmL) regions had the highest lignin concentration; however, even in this morphological region, the lignin concentration varied. Compared to lignin, cellulose distribution was more uniform; nevertheless, in specific limited areas, cellulose concentration varied significantly. At the present time the intensity maps of lignin and cellulose distributions are interpreted solely in terms of concentration differences but effect of orientation needs to be carefully considered in order to truly reveal the organization of the wood cell walls.

Keywords: Picea mariana, black spruce, cell walls, lignin, cellulose, raman imagery

File size: 1 kb(s)

This publication is also viewable on Treesearch:  view
RITS Product ID: 16711
Current FPL Scientist associated with this product
Agarwal, Umesh P.
Research Chemist
  

Additional items that might interest you
View the video celebrating FPL's 100 years of public service in 2010, from the producers of the Greatest Good....view

Research Highlights from FPL....view

Termite Eradication: A search for the Holy Grail.... view

Moisture Management in Residential Construction Series videos...view

Wood Floor Systems in Residential Construction Series videos....view
- FPL's Mission and Strategic Plan -

FPL's mission is to identify and conduct innovative wood and fiber utilization research that contributes to conservation and productivity of the forest resource, thereby sustaining forests, the economy, and quality of life. ... ..more »

- FPL Research Emphasis Areas -
Advanced Composites

As an integral part of the FPL mission, we improve the long-term sustainability of our Nation's forests by creating valuable composite products from biobased materials ... ..more »


Advanced Structures

The FPL has been in the forefront of wood-frame housing research since 1910 and has long been recognized as a world leader in such housing-related areas as engineered wood ... ..more »


Forest Biorefinery

We all know the compelling reasons that the United States needs to reduce its dependence on fossil fuels. Historically, the greatest increases in energy demand have been for transportation fuels ... ..more »


Nanotechnology

A leader in wood products research for over a century, the FPL is positioning itself to become the lead Federal research facility for the application of nanotechnology in forest products ... more »


Woody Biomass Utilization

Forests in the United States contain a substantial amount of small-diameter, overstocked, and underutilized material.FPL research projects are exploring the potential of the small-diameter ... ..more »