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Title: Improving the two-step remediation process for CCA-treated wood. Part II, Evaluating bacterial nutrient sources

Source: Waste management. Vol. 24 (2004): Pages 407-411

Author(s)Clausen, Carol A.

Publication Year: 2004  View PDF »

Category: Journal Articles

Abstract: Remediation processes for recovery and reuse of chromated-copper-arsenate-(CCA) treated wood are not gaining wide acceptance because they are more expensive than landfill disposal. One reason is the high cost of the nutrient medium used to culture the metal tolerant bacterium, Bacillus licheniformis, which removes 70-100% of the copper, chromium, and arsenic from CCA-treated southern yellow pine (CCA-SYP) in a two-step process involving oxalic acid extraction and bacterial culture. To reduce this cost, the nutrient concentration in the culture medium and the ratio of wood to nutrient medium were optimized. Maximum metal removal occurred when B. licheniformis was cultured in 1.0% nutrient medium and at a wood to nutrient medium ratio of 1:10. Also, malted barley, an abundant by-product of brewing, was evaluated as an alternative nutrient medium. Tests were done to determine absorption of metals by barley, and the results indicate that the barley acted as a biosorbent, removing heavy metals from the liquid culture after their release from CCA to SYP. For comparison, tests were also performed with no nutrient medium. Following bacterial remediation, 17% copper and 15% arsenic were removed from an aqueous slurry of CCA-SYP (no medium). When oxalic acid extraction preceded the aqueous bacterial culture, 21% copper, 54% chromium, and 63% arsenic were removed. The two-step process (oxalic acid extraction and bacterial culture with nutrient medium) appears to be an effective, yet costly, way to remove metals.

Keywords: Bacillus licheniformis; CCA; bioremiadiation; bacterial nutrient sources

File size: 644 kb(s)

This publication is also viewable on Treesearch:  view
RITS Product ID: 21921
Current FPL Scientist associated with this product
Clausen, Carol A.
Supervisory Research Microbiologist

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