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Title: A New Subfamily of Glycoside Hydrolase Family 30 with Strict Xylobiohydrolase Function

Source: Frontiers in Molecular Biosciences

Author(s)Crooks, Casey ; Bechle, Nathan J.; St John, Franz J.

Publication Year: 2021  View PDF »

Category: Journal Articles
Associated Research Project(s):   FPL-4712-3C

Abstract: The Acetivibrio clariflavus (basonym: Clostridium clariflavum) glycoside hydrolase family 30 cellulosomal protein encoded by the Clocl_1795 gene was highly represented during growth on cellulosic substrates. In this report, the recombinantly expressed protein has been characterized and shown to be a non-reducing terminal (NRT)-specific xylobiohydrolase (AcXbh30A). Biochemical function, optimal biophysical parameters, and phylogeny were investigated. The findings indicate that AcXbh30A strictly cleaves xylobiose from the NRT up until an α-1,2-linked glucuronic acid (GA)-decorated xylose if the number of xyloses is even or otherwise a single xylose will remain resulting in a penultimate GA-substituted xylose. Unlike recently reported xylobiohydrolases, AcXbh30A has no other detectable hydrolysis products under our optimized reaction conditions. Sequence analysis indicates that AcXbh30A represents a new GH30 subfamily. This new xylobiohydrolase may be useful for commercial production of industrial quantities of xylobiose.

Keywords: xylobiohydrolase; glycoside hydrolase; glycoside hydrolase family 30; AcXbh30A; Acetivibrio clariflavus; xylobiose; glucuronoxylan; Hungateiclostridium clariflavum

Publication Review Process: Formally Refereed

File size: 2,048 kb(s)

Date posted: 09/14/2021

This publication is also viewable on Treesearch:  view
RITS Product ID: 10250
Current FPL Scientists associated with this product (listed alphabetically)
Crooks, M.E. Casey
Research Microbiologist
St. Johns, Franz
Research Microbiologist
 

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