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Title: A study of the gut bacterial community of Reticulitermes virginicus exposed to chitosan treatment

Source: Insects. 11(10): 681. 17 p.

Author(s)Telmadarrehei, Telmah ; Tang, Juliet D.; Raji, Olanrewaju ; Rezazadeh, Amir ; Narayanan, Lakshmi ; Shmulsky, Rubin ; Jeremic, Dragica

Publication Year: 2020  View PDF »

Category: Journal Articles

Abstract: A thorough understanding of microbial communities in the gut of lower termites is needed to develop target-specifc and environmentally benign wood protection systems. In this study, the bacterial community from Reticulitermes virginicus was examined by Illumina sequencing of 16S ribosomal RNA (rRNA) spanning the V3 and V4 regions. Prior to library preparation, the termites were subjected to fve treatments over an 18-day period: three groups were fed on wood treated with 0.5% chitosan, 25% acetic acid, or water, the fourth group was taken directly from the original collection log, and the ffth group was starved. Metagenomic sequences were analyzed using QIIME 2 to understand the treatments’ effects on the dynamics of the gut bacteria. Four dominant phyla were detected: Bacteroidetes (34.4% of reads), Firmicutes (20.6%), Elusimicrobia (15.7%), and Proteobacteria (12.9%). A signifcant effect of chitosan treatment was observed in two phyla; Firmicutes abundance was signifcantly lower with chitosan treatment when compared to other groups, while Actinobacteria was lower in unexposed and starved termites. The results suggest that chitosan treatment not only affects the structure of the microbial community in the gut, but other treatments such as starving also cause shifts in termite gut communities.

Keywords: Chitosan; Reticulitermes virginicus; gut bacteria; 16S rRNA; Illumina amplicon sequencing; metagenomics

Publication Review Process: Formally Refereed

File size: 1,024 kb(s)

Date posted: 11/06/2020

This publication is also viewable on Treesearch:  view
RITS Product ID: 10059
Current FPL Scientist associated with this product
Tang, Juliet
Research Forest Products Technologist

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