Franz St. John
Franz St. JohnInstitute for Microbial and Biochemical Sciences
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Research in my laboratory focuses on the interface between the physical disruption and the subsequent enzyme hydrolysis stages necessary to prepare woody biomass for microbial conversion to biofuels and value-added products. Our guiding hypothesis is that through the development of better, more efficient enzyme systems, it may be possible to limit the chemical and physical input required during the initial disruption stage resulting in a reduced cost for microbial bioconversions of this important renewable resource.In this research our primary concern is the second most abundant but chemically most complex biomass polymer, the β-1,4 linked xylose polysaccharide 4-O-methyl glucuronoarabinoxylan (xylan). This polymer is important in that it associates through covalent attachment to interconnect the lignified cell wall with the rigid cellulose fibrils. The pretreatment process may be augmented with enzymes that target the specific linkages connecting lignin and xylan thereby facilitating the secondary cellulolytic enzyme processing and potentially limiting the initial physical pretreatment costs. Our research applies bacteriology, biochemistry, bioinformatic resources as well as structural biology to identify proteins which may function optimally to degrade minimally processed lignocellulosic biomass.A second goal of my laboratory is the development of second generation bacterial biocatalysts. Initial efforts will focus on defining and characterizing Gram positive bacterial characteristics that show promise in bioconversion processes. Examples of such characteristics include robust protein secretion and the potential of many Gram positive bacteria to transport complex oligosaccharides. Understanding how such physiological features function and how they can be employed in bacterial biocatalysts is of great value to the development of engineered biocatalyst.
- Microbiology and enzymology of lignocellulose bioconversion
- X-ray crystallographic protein structure studies of enzymes involved in biomass degradation
- Development of second generation bacterial biocatalysts with enhanced capabilities of protein secretion and complex carbohydrate utilization
- University of Maryland, Baltimore, Postdoctoral Fellowship X-ray Crystallography Core Facility , 2009
- University of Florida, Ph.D. Microbiology and Cell Science , 2006
- University of Florida, Gainesville, B.S. University of Florida, Gainesville , 1999
- American Society for Microbiology, Member
|Publication Year: 2016|
Highlight ID: 663
|Characterization of Microbial Biocatalysts in Lignocellulosic Utilization|
Bioconversion of lignocellulosic biomass would benefit from development of second generation bacterial biocatalysts. The bacterium Paenibacillus sp. strain JDR2, originally isolated from decaying sweetgum wood disks, has the ability to process a vari ...
Highlight ID: 662
|Development of Enzymes for use in Lignocellulose Processing|
The ultimate best use of lignocellulose allows for the selective extraction of defined value streams. To facilitate this, Forest Service research strives to develop new enzymes with unique capabilities in biomass processing. These new enzymes aim to ...
|Publication Year: 2015|
Highlight ID: 613
|Protein Structure and Biochemical Characterization of a Novel Functioning Xylanase|
Scientists identified and characterized a xylanase with unique function that may have applications in processing of woody biomass substrate. ...
|Publication Year: 2013|
Highlight ID: 452
|Researchers Determine the Structure of Bacterial Protein Involved in Biomass Conversion|
Researchers structurally characterized a bacterial protein involved in biomass degradation. The knowledge obtained from this novel protein suggest that it may function better than comparable enzymes in targeting complex lignocellulosic biomass. ...